20 ASSISTED OOCYTE ACTIVATION TO IMPROVE FERTILIZATION IN VITRO
نویسندگان
چکیده
منابع مشابه
Effects of assisted oocyte activation with calcium- ionophore and strontium chloride on in vitro ICSI outcomes
Objective(s): Failed fertilization after intra-cytoplasmic sperm injection (ICSI) is mainly attributed to failed oocyte activation and can be overcome by artificial oocyte activation (AOA). The present study aims to compare in vitro outcomes of ICSI following two different assisted oocyte activation chemical procedures (SrCl2 and Ionomycin) in sibling oocytes of ICSI candidates.Materials and Me...
متن کاملFertilization failure and oocyte activation.
Recent reports have suggested that when unfertilized oocytes with a spermatozoon after intracytoplasmic sperm injection (ICSI) are properly activated, the activated oocytes develop normally similar to fertilized oocytes. However, human oocytes do not easily respond to universal activators of mammalian oocytes like ethanol or calcium ionophore A23187, which induce a calcium transient in ooplasm....
متن کاملTreatment option for sperm- or oocyte-related fertilization failure: assisted oocyte activation following diagnostic heterologous ICSI.
BACKGROUND Failed fertilization occurs in 2-3% of ICSI cycles and is mainly due to lack of oocyte activation. Heterologous ICSI of patient's sperm in mouse oocytes allows discrimination between sperm- and oocyte-related aetiologies of activation failure. Assisted oocyte activation (AOA) by Ca-ionophore treatment can initiate fertilization in subsequent therapeutic ICSI. We report on diagnosis a...
متن کاملAssisted oocyte activation
Assisted oocyte activation (AOA) is the technique that simulates the effect of sperm penetration into an oocyte inducing oocyte activation. This technique is used in combination with intracytoplasmic sperm injection (ICSI) to improve the outcome of the fertilization process. It is mostly beneficial and recommended in those cases, when classic ICSI fails. This accounts approximately up to 5 % of...
متن کاملIn vitro oocyte maturation and fertilization.
Complete functional oocyte maturation involves many complex processes that occur within preovulatory follicles and are initiated and sustained by the gonadotropins, steroid hormones and many recognized and unrecognized factors. The product of oocyte maturation is a mature egg that has complete nuclear, plasma membrane and cytoplasmic changes that enable normal fertilization and subsequent devel...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2010
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)62438-8